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NNMT is overexpressed in aged skeletal muscle, where it has been linked to impaired NAD+ salvage, dysregulated sirtuin-1 activity, and increased senescence of muscle stem cells (satellite cells) — the cells responsible for muscle repair

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Routine aspiration is no longer recommended by the WHO or CDC for the deltoid, ventrogluteal or vastus lateralis sites, as those sites carry negligible vessel risk