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Some of the right lungs were harvested and stored at -80°C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining

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"Serum N(1)-Methylnicotinamide Is Associated With Obesity and Diabetes in Chinese." Journal of Clinical Endocrinology & Metabolism