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To refine the size and reduce polydispersity, extrusion or sonication steps are employed (Figure 2)

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LpdA is homologous to Gor and the mutant form of this protein may perform a similar function as Gor in order to reduce disulfide bonds in these suppressors, or may be able to reduce oxidized lipoamide and transfer electrons to ribonucleotide reductase via a novel mechanism