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The lack of LCMV-specific antibodies in our mutant mice could be caused by either a failure of the GC reaction or a defect in antibody production

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Primary antibody (anti-GCSc 1:10 (SantaCruz, sc390811) and anti-CD45 1:200 (Proteintech, 31243-1-AP)) incubation was carried out overnight at 4 °C